Fig 6.

Norepinephrine-induced PLC isozyme protein content in cardiomyocytes transfected with c-Fos and c-Jun siRNA. Representative blots (A) of PLC isozyme β₁ (150 kD), β₃ (150 kD) and δ₁ (85 kD) protein content and quantified data showing PLC β₁ (B), β₃ (C) and δ₁ (D) protein content (arbitrary densitometric units) in c-Fos and c-Jun siRNA (5 nM) transfected cardiomyocytes treated with NE (5 μM) for 24 hrs. Control cardiomyocytes were treated with 5 nM negative control siRNA as described in ‘materials and methods’ section. Values are mean ± S.E. of five experiments performed with five different cardiomyocyte preparations. *Significantly different (P < 0.05) versus control; ^#significantly different (P < 0.05) versus NE. CONT, control; NC, negative control; NE, norepinephrine; siRNA, small interfering RNA.