Fig 8.
Inhibition of norepinephrine-induced increases in ANF mRNA levels and protein synthesis in cardiomyocytes transfected with PLC isozyme siRNA. Representative blots (A) and quantified data showing ANF (161bp) mRNA levels relative to GAPDH (138bp) mRNA level in cardiomyocytes transfected with 5 nM PLC isozyme (B) β1, (C) β3, (D) γ1 and (E) δ1 siRNA and treated with NE (5 μM) for 2 hrs. Protein synthesis (F) was determined as incorporation of [3H] phenylalanine (DPM) into newly synthesized protein. Control cardiomyocytes were transfected with 5 nM negative control siRNA as described in ‘materials and methods’ section. Values are mean ± S.E. of five experiments performed with five different cardiomyocyte preparations. *Significantly different (P < 0.05) versus control; #significantly different (P < 0.05) versus NE. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; CONT, control; NC, negative control; NE, norepinephrine; siRNA, small interfering RNA.