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. 2013 Sep 27;288(46):32837–32851. doi: 10.1074/jbc.M113.486670

FIGURE 5.

FIGURE 5.

GFFD and GdFFD are detected from neurons isolated from the pedal ganglia from A. californica using LC-MS. A, LC separation of GFFD and GdFFD. B, MS/MS fragmentation spectrum of endogenous GFFD (21.4 min). C, MS/MS fragmentation spectrum of endogenous GdFFD (24.3 min). D, retention times on the LC and fragmentation patterns (E and F) of both peaks match well with those of synthetic peptides, which confirm the identification. E, MS/MS fragmentation spectrum of synthetic GFFD (21.4 min). F, MS/MS fragmentation spectrum of synthetic GdFFD (24.3 min).