FIGURE 3.

ISLAD CM peptides preferentially bind T-cells over B-cells. Splenocytes derived from C57BL/6J mice were incubated with rhodamine-labeled peptides. Thereafter, the cells were stained with either phycoerythrin-conjugated anti-CD3 or anti-B220 antibody and analyzed by flow cytometry. A, defining a lymphocyte gate using forward (FS) and side (SS) scatter analysis on splenocytes. B, defining a rhodamine-labeled lymphocyte gate. C, the percentage of T-cells and B-cells in the rhodamine-labeled cells was analyzed using anti-CD3 and anti-B220 antibody, respectively.