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Both ISLAD CM forms co-localize with the TCR. Activated mMOG(35–55) T-cells were probed with anti-TCRα antibody, followed by staining with FITC-labeled secondary antibody (left panels, green) and with rhodamine-labeled fluorescent peptides (ISLAD l-CM-Rho, ISLAD d-CM-Rho, and a control antimicrobial peptide (AMP-Rho); middle panels, red). The merged images of the molecules are shown (right panels). Scale bars = 2 μm.
