FIGURE 6.

Administering ISLAD d CM to EAE mice down-regulates pathogenic T-cells. A, ex vivo down-regulation of encephalitogenic MOG(35–55) T-cells following ISLAD d-CM administration. C57BL/6J mice were injected for EAE induction with the MOG(35–55) peptide. Ten days after immunization, draining LNCs from each treatment group (n = four mice per group) were pooled and analyzed for their ex vivo recall proliferative response to MOG(35–55). The LNCs from each treatment group (ISLAD d-CM or PBS) were cultured for 72 h in microtiter wells in triplicate (0.5 × 10⁶/well) in the absence or presence of MOG(35–55) (5 μg/ml). [³H]Thymidine was added for the last 18 h. The results are expressed as the stimulation index (S.I; mean cpm of antigen-containing cultures/mean cpm of medium-containing cultures). B and C, secretion of proinflammatory cytokines from cultured LNCs derived from PBS- or ISLAD d-CM-administered EAE mice. LNCs from each treatment group were cultured (5 × 10⁶/ml) in the absence or presence of MOG(35–55) (5 μg/ml) for 24 h, and the supernatants were collected to detect the secreted IFNγ (B) and IL-17 (C) by ELISA. The results are the mean ± S.D. from a representative experiment. *, p < 0.05.