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Functional characterization of the C-terminal helix deletion of CagF. A, whole-cell lysates of the indicated H. pylori strains were subjected to CagA immunoprecipitation. Extracts and IP fractions were analyzed by Western blot for CagA and CagF. B, AGS cells were infected for 4 h with the indicated strains or left uninfected. Infection lysates were analyzed by Western blot (WB) with CagA- and phosphotyrosine (PTyr)-specific antibodies.
