TABLE 2.

Thermodynamic parameters of binding between CagA_WT and CagF variants

All values are means of duplicate runs.

CagF mutation	N	Kd	ΔH	ΔS	TΔS
		nm	kcal mol−1	cal K−1 mol−1	kcal mol−1
WT	0.98 ± 0.02	49 ± 1	−38.4 ± 1.1	−95.2 ± 3.7	−28.4
F30A	0.92 ± 0.02	35 ± 4	−37.4 ± 0.6	−91.2 ± 1.5	−27.2
E31A	1.11 ± 0.01	43 ± 12	−28.1 ± 1.5	−60.5 ± 5.7	−18.0
L32A	1.11 ± 0.02	105 ± 12	−32.6 ± 0.3	−77.2 ± 0.5	−23.0
K33A	1.10 ± 0.01	61 ± 1	−33.2 ± 0.1	−78.4 ± 0.3	−23.4
E34A	1.09 ± 0.00	61 ± 4	−32.6 ± 1.4	−76.4 ± 4.9	−22.8
E35A	0.95 ± 0.03	92 ± 30	−34.0 ± 1.4	−81.5 ± 5.2	−24.3
L36A	1.22 ± 0.03	1500 ± 16	−25.0 ± 0.6	−57.3 ± 2.1	−17.1
D37A	0.98 ± 0.02	136 ± 20	−34.0 ± 1.6	−82.6 ± 5.7	−24.6
F38A	0.95 ± 0.00	107 ± 18	−30.5 ± 0.4	−70.3 ± 1.6	−21.0
I39A	1.16 ± 0.02	793 ± 84	−31.0 ± 0.2	−75.7 ± 0.7	−22.6
E40A	1.05 ± 0.04	384 + 21	−31.1 ± 0.4	−74.9 ± 1.1	−22.3