TABLE 3.
Comparison of the thermodynamic parameters of binding between CagF and CagFS234Stop and CagA variants
All values are means of duplicate runs.
| N | Kd | ΔH | ΔS | TΔS | |
|---|---|---|---|---|---|
| nm | kcal mol−1 | cal K−1 mol−1 | kcal mol−1 | ||
| CagAWT-CagFWT | 0.98 ± 0.02 | 49 ± 1 | −38.4 ± 1.1 | −95.2 ± 3.7 | −28.4 |
| CagAWT-CagFS234Stop | 0.99 ± 0.01 | 684 ± 51 | −27.0 ± 0.7 | −62.5 ± 2.5 | −18.6 |
| CagA1–885-CagFWT | 1.16 ± 0.03 | 16,000 ± 500 | −16.4 ± 0.7 | −33.0 ± 2.2 | −10.5 |
| CagA1–885-CagFS234Stop | 0.99 ± 0.02 | 7,000 ± 1,600 | −3.5 ± 0.2 | +12.4 ± 0.7 | +3.7 |
| CagA1–1054-CagFWT | 0.86 ± 0.04 | 4,600 ± 430 | −7.3 ± 0.7 | +0.2 ± 2.4 | +0.1 |
| CagA1–1054-CagFS234Stop | 0.89 ± 0.04 | 2,900 ± 340 | −3.9 ± 0.1 | +12.3 ± 0.1 | +3.7 |
| CagA1055–1247-CagFWT | 47,000 ± 6,000 | ||||
| CagA1055–1247-CagFS234Stop | 25,000 ± 3,600 |