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. 2013 Sep 19;288(46):32979–32990. doi: 10.1074/jbc.M113.483123

FIGURE 7.

FIGURE 7.

Peptides derived from neutrophil enzyme-revealed tethered ligands activate PAR1-dependent MAP kinase signaling in a Gαi-dependent manner. A, neutrophil elastase-tethered ligand (NE-TL-AP, RNPNDKYEPF-NH2), and B, proteinase-3-tethered ligand (PR3-TL-AP, TLDPRSF-NH2) peptides activate KNRK-PAR1-eYFP cell MAPK in a concentration-dependent manner. MAPK activation is not seen in empty vector (EV)-transfected KNRK-pCDNA3 cells treated with 20 μm NE-TL-AP or PR3-TL-AP. C, MAPK activation by NE-TL-AP, and D, PR3-TL-AP in endogenous PAR1-expressing HEK-293 cells, but not in HEK-293 cells treated with the PAR1 antagonist SCH79797 (2 μm). E, histogram quantifying the PTX inhibition of MAPK signaling caused by thrombin, NE-TL-AP, and PR3-TL-AP in KNRK-PAR1-eYFP cells. Data are expressed as mean ± S.E. * indicates significant difference (p < 0.05, n = 3) from non-PTX-treated cell MAPK signal.