Effect of knockdown of different mouse Slfns on cell proliferation of malignant melanoma cells.
A, B16-F1 cells were transfected with control siRNAs or siRNAs specific for Slfn2, Slfn3, or Slfn5 as indicated. 48 h post-transfection, an equal number of cells was plated and allowed to proliferate for 72 h, at which time point the cells were counted. Data are expressed as % control siRNA samples and represent means ± S.E. of four experiments. *, p < 0.05 versus control siRNA cells. B–D, B16-F1 cells transfected with siRNAs against the indicated Slfn genes were treated with indicated doses of IFNα, and proliferation was measured by MTT assays. Data are expressed as % untreated (UT) control (CTL) siRNA samples and represent means ± S.E. of the several experiments. *, p < 0.05, Slfn2 (n = 5), Slfn3 (n = 5), and Slfn5 (n = 3). Und, undetected.