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. 2013 Oct 2;288(46):33006–33015. doi: 10.1074/jbc.M113.460741

FIGURE 4.

FIGURE 4.

Effects of knockdown of different Slfn genes on anchorage-independent malignant melanoma cell growth. A, B16-F1 cells were transfected with control siRNA or Slfn2 siRNA. Equal number of cells were plated in soft agar and allowed to form colonies for 14 days. Representative images of the soft agar wells are shown. B, quantitation of colonies from four independent experiments, including the one shown in A. Data are expressed as % control siRNA transfected cells and represent means ± S.E. of four experiments. *, p < 0.05. C, B16-F1 cells were transfected with control siRNA or Slfn3 or Slfn5 siRNAs. An equal number of cells was plated in soft agar and allowed to form colonies for 14 days. Representative images of the soft agar wells are shown. D, quantitation of colonies from four independent experiments, including the one shown in C. Data are expressed as % control siRNA transfected cells and represent means ± S.E. of four experiments. *, p < 0.05. E, clone M3 cells were transfected with control (CTL) siRNA or Slfn3 or Slfn5 siRNAs. An equal number of cells was plated in soft agar, and colonies from four independent experiments were quantified after 14 days. Data are expressed as % control siRNA-transfected cells and represent means ± S.E. of four experiments. *, p < 0.05.