FIGURE 7.

Effects of knockdown of different mouse Slfns on cell proliferation of mouse renal cell carcinoma cells. A, RENCA cells were transfected with control siRNAs or siRNAs specific for Slfn2, Slfn3, or Slfn5 as indicated. 48 h post-transfection, an equal number of cells was plated and allowed to proliferate for 72 h, at which time the cells were counted. Data are expressed as % of control (CTL) siRNA-transfected samples and represent means ± S.E. of three experiments. *, p < 0.05 versus control siRNA cells. B, transfected RENCA cells were treated with indicated doses of IFNα, and viability was assessed by MTT assays. Data are expressed as % untreated control (CTL) siRNA samples and represent means ± S.E. of five experiments. *, p < 0.05 control versus Slfn2, control versus Slfn5, **, p < 0.05 control versus Slfn2, control versus Slfn3, control versus Slfn5.