FIGURE 9.

Effects of overexpression of mouse Slfn2 on cell proliferation and anchorage-independent growth in kidney carcinoma cells. A and B, CD3575 cells were transiently transfected with either empty vector (E.V.) or Slfn2 cDNA. Levels of Slfn2 were quantified at 48 h post-transfection by quantitative RT-PCR (A) or examined by immunoblotting (B). The quantitative RT-PCR data are expressed as fold increase in the Slfn2-transfected samples over empty vector transfected samples normalized to GAPDH and represent means ± S.E. from two independent experiments performed in triplicate. C, CD3575 cells were transiently transfected with either empty vector (E.V.) or Slfn2 cDNA. An equal number of cells was plated in soft agar and allowed to form colonies for 8 days. Representative images of the soft agar wells are shown. D, quantitation of colonies from three independent experiments, including the one shown in A. Data are expressed as % control empty vector transfected cells and represent means ± S.E. of three experiments performed in triplicate. *, p < 0.05.