FIGURE 5.

miR-146a modulates SHH signaling by targeting NUMB. A–C, pGVP2-NUMB 3′-UTR luciferase reporter construct or pGVP2-NUMB 3′-UTR reverse luciferase reporter construct (3′-UTR is in reverse orientation)-transfected macrophages were treated with MDP (A) or SIN1 (B) or co-transfected with miR-146a mimics (C) to analyze NUMB 3′-UTR luciferase activity (mean ± S.E., n = 3) (one-way ANOVA) or NUMB protein levels. Med, medium; RLU, relative luciferase unit. D and E, miR-146a mimic-transfected macrophages were assessed for activation of SHH signaling at protein (D) and transcript levels (E).