TABLE 1.
Interacting molecular pairs and conditions | Cumulative probability |
Figure(s) | |
---|---|---|---|
Forces >10 pN | Forces >60 pNa | ||
% | % | ||
Uncross-linked WT PF4b and KKO | 13.2 ± 2.4 | 0.5 ± 0.1 | 1A |
Uncross-linked WT PF4b and RTO | 7.4 ± 1.4 | 0.4 ± 0.1 | 1B |
Uncross-linked WT PF4b and Fab KKO | 11.3 ± 2.3 | 0.5 ± 0.1 | S6 |
(Uncross-linked WT PF4b + PF4A01) and KKO | 10.7 ± 2.4 | 0.8 ± 0.3 | S7A |
(Uncross-linked WT PF4b + PF4A01) and RTO | 5.2 ± 1.1 | 0.3 ± 0.1 | S7B |
Cross-linked WT PF4 (0.1 mg/ml) and KKO | 9.2 ± 2.7 | 2.4 ± 0.4 | S9A |
Cross-linked WT PF4 (0.2 mg/ml) and KKO | 14.6 ± 2.5 | 6.2 ± 0.9 | S7B |
Cross-linked WT PF4 (1 mg/ml) and KKO | 15.2 ± 3.4 | 9.6 ± 1.8 | 2A and S9C |
Cross-linked WT PF4 (2 mg/ml) and KKO | 19.0 ± 2.8 | 10.2 ± 1.2 | S9D |
Cross-linked WT PF4b and RTO | 4.6 ± 1.0 | 1.2 ± 0.5 | 2B |
Cross-linked WT PF4b and Fab KKO | 9.9 ± 2.2 | 6.5 ± 1.5 | S8 |
(Cross-linked WT PF4b + free Fab KKO) and KKO | 7.4 ± 1.2 | 1.1 ± 0.3 | 2C |
Cross-linked WT PF4b and (KKO + free PF4) | 29.2 ± 4.5 | 0.9 ± 0.4 | 2D |
Uncross-linked PF4 K50Eb and KKO | 17.2 ± 3.6 | 0.3 ± 0.1 | 5A |
Uncross-linked PF4 K50Eb and RTO | 14.1 ± 2.1 | 0.3 ± 0.1 | 5B |
Uncross-linked PF4 K50Eb and Fab KKO | 12.7 ± 3.1 | 0.4 ± 0.1 | S10 |
Cross-linked PF4 K50Eb and KKO | 9.4 ± 2.1 | 3.2 ± 1.1 | 6A |
Cross-linked PF4 K50Eb and RTO | 4.4 ± 1.1 | 2.0 ± 0.6 | 6A |
Cross-linked PF4 K50Eb and Fab KKO | 8.3 ± 2.4 | 1.2 ± 0.4 | S11 |
Uncross-linked WT PF4b-heparin and KKO | 18.9 ± 3.7 | 5.5 ± 1.1 | 7A |
Uncross-linked WT PF4b-heparin and RTO | 8.0 ± 2.2 | 0.4 ± 0.1 | 7B |
Cross-linked WT PF4b-heparin and KKO | 15.4 ± 3.3 | 11.8 ± 2.3 | 7C |
Cross-linked WT PF4b-heparin and RTO | 6.6 ± 2.0 | 3.3 ± 0.8 | 7D |
a Rupture forces of >60 pN have been identified as reflecting specific antigen-antibody interaction between PF4 and KKO or RTO.
b To keep the surface density the same, the concentration of PF4 in the immobilizing mixture was 1 mg/ml, unless otherwise specified.