TABLE 1.

Cumulative binding probability for different interacting proteins at various conditions

Values are expressed as means ± S.D.

Interacting molecular pairs and conditions	Cumulative probability		Figure(s)
	Forces >10 pN	Forces >60 pNa
	%	%
Uncross-linked WT PF4b and KKO	13.2 ± 2.4	0.5 ± 0.1	1A
Uncross-linked WT PF4b and RTO	7.4 ± 1.4	0.4 ± 0.1	1B
Uncross-linked WT PF4b and Fab KKO	11.3 ± 2.3	0.5 ± 0.1	S6
(Uncross-linked WT PF4b + PF4A01) and KKO	10.7 ± 2.4	0.8 ± 0.3	S7A
(Uncross-linked WT PF4b + PF4A01) and RTO	5.2 ± 1.1	0.3 ± 0.1	S7B
Cross-linked WT PF4 (0.1 mg/ml) and KKO	9.2 ± 2.7	2.4 ± 0.4	S9A
Cross-linked WT PF4 (0.2 mg/ml) and KKO	14.6 ± 2.5	6.2 ± 0.9	S7B
Cross-linked WT PF4 (1 mg/ml) and KKO	15.2 ± 3.4	9.6 ± 1.8	2A and S9C
Cross-linked WT PF4 (2 mg/ml) and KKO	19.0 ± 2.8	10.2 ± 1.2	S9D
Cross-linked WT PF4b and RTO	4.6 ± 1.0	1.2 ± 0.5	2B
Cross-linked WT PF4b and Fab KKO	9.9 ± 2.2	6.5 ± 1.5	S8
(Cross-linked WT PF4b + free Fab KKO) and KKO	7.4 ± 1.2	1.1 ± 0.3	2C
Cross-linked WT PF4b and (KKO + free PF4)	29.2 ± 4.5	0.9 ± 0.4	2D
Uncross-linked PF4 K50Eb and KKO	17.2 ± 3.6	0.3 ± 0.1	5A
Uncross-linked PF4 K50Eb and RTO	14.1 ± 2.1	0.3 ± 0.1	5B
Uncross-linked PF4 K50Eb and Fab KKO	12.7 ± 3.1	0.4 ± 0.1	S10
Cross-linked PF4 K50Eb and KKO	9.4 ± 2.1	3.2 ± 1.1	6A
Cross-linked PF4 K50Eb and RTO	4.4 ± 1.1	2.0 ± 0.6	6A
Cross-linked PF4 K50Eb and Fab KKO	8.3 ± 2.4	1.2 ± 0.4	S11
Uncross-linked WT PF4b-heparin and KKO	18.9 ± 3.7	5.5 ± 1.1	7A
Uncross-linked WT PF4b-heparin and RTO	8.0 ± 2.2	0.4 ± 0.1	7B
Cross-linked WT PF4b-heparin and KKO	15.4 ± 3.3	11.8 ± 2.3	7C
Cross-linked WT PF4b-heparin and RTO	6.6 ± 2.0	3.3 ± 0.8	7D

^a Rupture forces of >60 pN have been identified as reflecting specific antigen-antibody interaction between PF4 and KKO or RTO.

^b To keep the surface density the same, the concentration of PF4 in the immobilizing mixture was 1 mg/ml, unless otherwise specified.