FIGURE 3.

Concentrations of PK-d₇ (A), MK-4-d₇ (B), their respective epoxides, and MD-d₇ (C) in serum collected by heart puncture 6 h postadministration from cannulated rats orally administered PK-d₇ as a single dose of 10 μmol/kg body of weight are shown. PK-d₇, MK-4-d₇, and their epoxides in the samples were measured by APCI-LC-MS/MS as described under “Experimental Procedures.” MD-d₇ was measured by in vitro bioassay using rhUBIAD1 with GGPP-d₅ as described under “Experimental Procedures.” Concentrations of PK-d₇, MK-4-d₇, and their respective epoxides in small intestine (D), liver (E), heart (F), and cerebrum (G) collected 6 h postadministration from cannulated rats orally administered PK-d₇ as a single dose of 10 μmol/kg of body weight are shown. PK-d₇, MK-4-d₇, and their respective epoxides in tissues were measured by APCI-LC-MS/MS as described under “Experimental Procedures.” Except for the TLD experiment, which included three rats, the results represent the means for four rats (values are shown as columns) and the standard errors (vertical bars). The abbreviations BD, TLD, PV, and IVC represent bile duct-, thoracic lymph duct-, portal vein-, and inferior vena cava-cannulated rats, respectively.