FIGURE 1.
The CT TCR binds AH1·H-2Ld with increased affinity relative to the 1D4 TCR. Sf9 insect cells were infected with recombinant BV expressing transmembrane CT TCR (A) or 1D4 TCR (B). Three days postinfection, the cells were stained with increasing concentrations of H-2Ld tetramer loaded with the indicated peptide and TCRβ-specific antibody (clone HAM-597). The geometric MFI of tetramer staining was normalized to TCR expression and plotted. Combined data from three independent experiments are shown. C and D, insect cells were infected with recombinant BV expressing transmembrane pMHC, as indicated and stained with soluble CT TCR (C) or 1D4 TCR (D) and an MHC-specific antibody (clone 28.14.8s). Geometric MFI of TCR staining is shown after normalizing for MHC expression levels (38). Data shown are representative of three independent experiments with similar results.