FIGURE 10.

CPM increased agonist-mediated conformational change of the B1R. A, HEK cells stably expressing B1R-TC-CFP alone or stably co-expressing CPM were stimulated with 10 nm DAKD, and the change in intramolecular FRET was determined as described under “Experimental Procedures.” FU, fluorescent units. B, HEK cells stably expressing B1R-TC-Rluc alone or stably co-expressing CPM were stimulated with 10 nm DAKD, and the change in net BRET was determined. The data are expressed as the mean ± S.E. (n = 3). * = p < 0.05 versus Vehicle; # = p < 0.05 versus control without CPM (Student's t test).