FIGURE 6.

Effect of cellular dNTP on in vivo HIV-1 LTR 5′-end DNA gap repair in human primary macrophages. A, diagram for the experimental procedure. Human primary macrophages from four donors were transduced with the pD3HIV-GFP vector for 2 days. The transduced macrophages were then washed and treated with 1 μm raltegravir (Ralt.) and 5 μm nevirapine (Nev.) for 6 h. The treated macrophages were cultured for 96 h in the presence and absence of 1 mm dNs, and the cells were collected at 0, 24, 48, 72, and 96 h. B, quantitative AluI PCR. Genomic DNAs were prepared from the collected macrophages and analyzed by quantitative AluI PCR for monitoring the HIV gap DNA repair at the integration sites.