FIGURE 3.

PKAR response to glucose is altered by tyrosine kinases in Min6 cells. Effect of PKAR mutations that disrupt PKM2 inactivation by tyrosine kinases, quantified as % change in FRET activation from 0 to 25 mm glucose. Y105F blocks tyrosine phosphorylation of PKM2 (47), K433E blocks phosphotyrosine peptide binding by PKM2 (43), and S437Y blocks the ability of PKM2 to displace phosphotyrosine peptides (48). Error bars in all panels represent the mean ± S.E., n ≥ 33 cells per treatment from ≥2 independent experiments. Analysis of variance with Bonferroni post-test: **, p < 0.01; ***, p < 0.0001.