TABLE 1.

Effect of linker length on PKAR dynamic range

Linker amino acid composition is: 5, lsrat; 7, gtgggas; 28, sgtlvklstslykkagfkgtnsvdrtsaa; 22, lsryldpaflykvvdlegprat. Data are unpaired t test of 2.5 G vs. 25 G.

	%E, 2.5 G	%E, 25 G	Δ	%
Multi-chain PKAR
Cer-28-PKM2/Cit-28-PKM2 (1.0)	9.3 ± 0.2 (n = 75)	10.5 ± 0.3 (n = 61)	1.2	12.9%a
Cer-7-PKM2/Cit-7-PKM2 (1.0ΔN)	7.6 ± 0.4 (n = 22)	9.3 ± 0.7 (n = 22)	1.7	22.4%b
PKM2–22-Cer/PKM2–22-Cit (1.1)	7.7 ± 0.2 (n = 55)	8.2 ± 0.2 (n = 50)	0.5	6.5%b
PKM2–5-Cer/PKM2–5-Cit (1.1ΔC)	7.8 ± 0.2 (n = 21)	7.5 ± 0.2 (n = 23)	−0.3	−4.8%
Cer-28-PKM2/PKM2–22-Cit (1.2)	8.8 ± 0.3 (n = 84)	11.0 ± 0.4 (n = 76)	2.2	25.0%c
Cer-7-PKM2/PKM2–22-Cit (1.2ΔN)	6.5 ± 0.3 (n = 12)	8.5 ± 0.4 (n = 18)	2.0	30.7%c
Single-chain PKAR
Cit-28-PKM2–22-Cer (2.0)	25.1 ± 0.3 (n = 68)	27.3 ± 0.4 (n = 77)	2.2	8.7%c
Cit-7-PKM2–22-Cer (2.0ΔN)	28.6 ± 0.5 (n = 27)	31.8 ± 0.6 (n = 27)	3.2	11.1%c
Cit-28-PKM2–5-Cer (2.0ΔC)	32.6 ± 0.5 (n = 27)	33.9 ± 0.6 (n = 31)	1.3	4.0%
Cit-7-PKM2–5-Cer (2.0ΔNΔC)	36.4 ± 0.4 (n = 76)	37.4 ± 0.4 (n = 76)	1.0	3.0%

^a , p < 0.01;

^b, p < 0.05;

^c, p < 0.0001.