TABLE 1.

SUB1/Cond sporozoites are unable to establish a blood parasitemia

Groups of 4–5 mice were infected intravenously by SUB1/Cond or CONT sporozoites to determine the prepatent period before the establishment of a blood parasitemia. The number of animals in each group that were positive for erythrocytic stages is shown. Asterisks indicate that the analysis of genomic DNA prepared from blood stage parasites collected from these mice has shown that only the non-excised (NE) genotype for the Pbsub1 locus could be detected. PCR genotyping of the resulting erythrocytic SUB1/Cond parasites has been performed on parasites prepared from infected mice labeled with an asterisk. A representative PCR analysis showing that only the SUB1/Cond non-excised genotype could be detected and corresponding to the erythrocytic parasites prepared from the two infected mice (Experiment 6) is shown in Fig. 2F.

Experiment	Parasite clone	No. of injected sporozoites	No. of positive mice/no. of injected mice (blood parasitemia)	Prepatent period (days)	Genotype of recovered parasites
1	SUB1/Cond-22	30,000	2*/10	6	NE Pbsub1
	CONT		10/10	4	WT
2	SUB1/Cond-22	30,000	0/3
	CONT		3/3	5	WT
3	SUB1/Cond-22	30,000	2*/4	6	NE Pbsub1
	CONT		4/4	6	WT
4	SUB1/Cond-5	30,000	1*/5	7	NE Pbsub1
	CONT		5/5	6	WT
5	SUB1/Cond-5	30,000	2*/4	4	NE Pbsub1
	CONT		4/4	4	WT
6	SUB1/Cond-5	30,000	2*/5	4	NE Pbsub1
	CONT		5/5	4	WT