FIGURE 9.

Deletion mutants of miR-29 binding sites. Putative miR-29 binding sites (marked with ± in Fig. 8) in the miR-29 target genes were deleted from pMIR-REPORT Luciferase vectors. Luciferase activity was quantified in the RAW264.7 cells co-transfected with a miR-29c inhibitor or a scrambled nontargeting control and normalized to β-galactosidase activity. Cells were treated with RANKL (30 ng/ml) for 48 h after transfection. *, significantly different from scrambled; #, significantly different from 29c inhibitor in the wild-type vector, p < 0.05 (n = 6). CDS, coding sequence. A, calcitonin receptor gene; B, Cdc42 coding sequence; C, Srgap2; D, Gpr85; E, Cd93; F, Nfia.