TABLE 1.

Actin-activated MgATPase activity of full-length NM IIs and their HMM counterparts

The actin-activated MgATPase activity was measured at 25 °C as described under “Experimental Procedures.” HMM data from previously published papers are shown for comparison.

Paralog	Ref.	Vmax	KATPase
		s−1	μm
NM IIA		0.41 ± 0.04	3.2 ± 0.1
IIA HMM	79	0.45 ± 0.03	9.0 ± 2.0
NM IIB		0.23 ± 0.03	4.0 ± 0.3
IIB HMM	20	0.17 ± 0.04	3.4 ± 1.8
NM IIB2		0.04 ± 0.004	2.5 ± 0.3
IIB2 HMM	76	0.01 ± 0.003	2.8 ± 0.8
NM IIC		0.13 ± 0.02	1.6 ± 0.1
II C HMM	20	0.13 ± 0.04	4.2 ± 1.1