Reduced E2a dose results in impaired development of early progenitors.
A, diagrams showing the percentages or absolute numbers of phenotypic hematopoietic progenitor cells in Wt, Pax5+/−Ebf1+/−, E2a+/−, or Ebf1+/−E2a+/− mice of the total number of propidium iodide negative (live) cells in mouse BM. LSK cells are defined as Lin-SCA-1highKIThigh cells, while LMPPs are defined as FLT3highLSK cells. B, CLPs are identified as Lin−SCA-1low/intKITlow/intFLT3+IL7R+. These cells were then further subdivided based on the expression of Ly6D on the cell surface. Each dot represents one mouse, and the data are presented as median (horizontal line) ± interquartile range. Statistical analysis was performed using the Mann-Whitney U test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.