FIGURE 1.

Reduced E2a dose results in impaired development of early progenitors. A, diagrams showing the percentages or absolute numbers of phenotypic hematopoietic progenitor cells in Wt, Pax5^+/−Ebf1^+/−, E2a^+/−, or Ebf1^+/−E2a^+/− mice of the total number of propidium iodide negative (live) cells in mouse BM. LSK cells are defined as Lin-SCA-1^highKIT^high cells, while LMPPs are defined as FLT3^highLSK cells. B, CLPs are identified as Lin⁻SCA-1^low/intKIT^low/intFLT3⁺IL7R⁺. These cells were then further subdivided based on the expression of Ly6D on the cell surface. Each dot represents one mouse, and the data are presented as median (horizontal line) ± interquartile range. Statistical analysis was performed using the Mann-Whitney U test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.