FIGURE 6.

Heterozygote loss of Ebf1 results in disruption of cell cycle dynamics in early progenitor cells. A, diagrams indicating the median fluorescence intensity of AnnexinV on Pro-B (Lin⁻B220⁺CD19⁺CD43^highIgM⁻), Pre-B (Lin⁻B220⁺CD19⁺CD43^low/negIgM⁻), or IgM⁺ (Lin⁻B220⁺CD19⁺CD43^low/negIgM⁺) BM cells from Wt or Ebf1^+/− mice. B, cell cycle analysis of pro-B (Lin⁻B220⁺CD19⁺CD43^highIgM⁻) and pre-B (Lin⁻B220⁺CD19⁺CD43^low/negIgM⁻) cells from Wt and Ebf1^+/− BM based on expression of the intracellular proliferation marker Ki-67 and the DNA-staining dye DAPI. Cells negative for Ki-67 and with DAPI staining corresponding to that of a diploid cell were classified as being in G₀. Expression of Ki-67 and a normal DNA amount identify cells in G₁, whereas high DNA content in combination with Ki-67 expression identifies cells in S/G₂/M phase. Each dot in the panels represents one mouse. Data are presented as median (horizontal line) ± interquartile range. Statistical analysis was performed using the Mann-Whitney U test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.