Heterozygote loss of Ebf1 results in disruption of cell cycle dynamics in early progenitor cells.
A, diagrams indicating the median fluorescence intensity of AnnexinV on Pro-B (Lin−B220+CD19+CD43highIgM−), Pre-B (Lin−B220+CD19+CD43low/negIgM−), or IgM+ (Lin−B220+CD19+CD43low/negIgM+) BM cells from Wt or Ebf1+/− mice. B, cell cycle analysis of pro-B (Lin−B220+CD19+CD43highIgM−) and pre-B (Lin−B220+CD19+CD43low/negIgM−) cells from Wt and Ebf1+/− BM based on expression of the intracellular proliferation marker Ki-67 and the DNA-staining dye DAPI. Cells negative for Ki-67 and with DAPI staining corresponding to that of a diploid cell were classified as being in G0. Expression of Ki-67 and a normal DNA amount identify cells in G1, whereas high DNA content in combination with Ki-67 expression identifies cells in S/G2/M phase. Each dot in the panels represents one mouse. Data are presented as median (horizontal line) ± interquartile range. Statistical analysis was performed using the Mann-Whitney U test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.