FIGURE 9.

Heterozygote deletion of Ebf1 results in a reduction of pre-BCR expression on B-cell progenitors. Representative histograms (A) and diagrams (B) display expression of a λ5 promoter-regulated human CD25 (hCD25) reporter transgene on B-lineage populations from Wt or Ebf1^+/− mice. C, frequency of pre-BCR⁺ cells and median pre-BCR expression levels on (Lin⁻B220⁺CD19⁺CD43^low/negIgM⁺) cells; D, expression levels of IgM on pre-BCR⁺ cells from Wt and Ebf1^+/− BM. In the diagrams, each dot represents one mouse, and data are presented as median ± interquartile range. Statistical analysis was performed using the Mann-Whitney U test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.