TABLE 2.

Gene function enrichment analysis shows changes of the genes for cell division and mitosis in pre-B-cells from Ebf1^+/− mice

The table displays the average relative expression values as estimated by dCHIP analysis for a set of B-lineage genes in sorted pre-B (Lin⁻B220⁺CD19⁺CD43^low/negIgM⁻) cells from Wt or Ebf1^+/− mice. The lower part of the table displays gene ontology analysis of microarray data from pre-B-cells from either Wt or Ebf1^+/− mice. The data were generated by hybridization of cRNA to Affymetrix^TM microarrays and collected from two independently sorted populations. The analysis was done using dCHIP based on the genes that are 1.5-fold differentially expressed between Wt and Ebf1^+/− pre-B-cells.

mRNA	Wt CD43low/neg IgM−	Ebf1+/− CD43low/negIgM−	Change
			-fold
B-lineage genes
Ebf1	1459	973	−1.5
Cd79b	6879	5450	−1.3
Rag2	223	100	−2.2
Vpreb3	3205	1775	−1.8
Rag1	175	106	−1.6
Pax5	131	118	−1.1
Vpreb1///Vpreb2	64	56	−1.1
CD19	2580	2397	−1.1
Blnk	2549	2301	−1.1
IgH-V J558	1407	336	−4.2
Il2ra	46	23	−2.0
Il7ra	1123	691	−1.6
Gene ontology cell division (p = 0.000388)
CcnB1-rs1	56	21	−2.6
Cdc45l	60	22	−2.7
Cdc6	80	27	−3.0
Cyclin B1	149	66	−2.3
Racgap1	70	25	−2.8
Incemp	99	40	−2.5
Zc3hc1	106	55	−1.9
Kntc1	78	28	−2.8
Lig4	344	102	−3.4
Ube2c	181	68	−2.6
Rasgef1b	9	42	−4.4
Gene ontology mitosis (p = 0.000479)
CcnB1-rs1	56	21	−2.6
Cdc6	80	27	−3.0
Racgap1	70	25	−2.8
Incemp	99	40	−2.5
Zc3hc1	106	55	−1.9
Kntc1	78	28	−2.8
Plk1	83	35	−2.3
Ube2c	181	68	−2.6