Skip to main content
. 2013 Oct 6;288(46):33470–33482. doi: 10.1074/jbc.M113.496497

TABLE 2.

Kinetics data for the oxidation of TCP as catalyzed by DHP in the ferric state in the presence of H2O2 at pH 7

Protein KmH2O2 kcat kcat/KmH2O2
μm s1 μm1s1
DHP A (WT) 23 ± 1 0.61 ± 0.01 0.027
DHP A (Y34F) 27 ± 5 0.77 ± 0.02 0.029
DHP A (Y38F) 68 ± 5 1.09 ± 0.05 0.016
DHP A (Y34F/Y38F) 56 ± 7 4.9 ± 0.1 0.09
DHP B (WT) 22 ± 2 1.53 ± 0.03 0.070
DHP B (Y28F) 752 ± 48 5.13 ± 0.14 0.007
DHP B (Y38F) 60 ± 10 8.53 ± 0.33 0.143
DHP B (Y28F/Y38F) 47 ± 5 5.37 ± 0.07 0.114