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A, representation of interdomain interaction between R815A of CL3 region and Asp-522 of NBD1 domain. B, immunoblot of whole cell lysates (100 μg of protein per lane) prepared from NIH3T3 cells transfected with human ABCC4 WT and ABCC4 mutant (R815A) and incubated at 37 or 28 °C for 24 h after 24 h of transfection. C, densitometric quantification of the blots.
