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. 2013 Jun 19;288(31):22270–22280. doi: 10.1074/jbc.M113.475558

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FIGURE 2. — All eight N-glycosylation sites of recombinant rKOR1 harbor glycans. Spectra were obtained by LC-ESI-MS analysis of trypsin/chymotrypsin double-digested rKOR1 WT purified from insect culture supernatants. The major peaks with masses corresponding to individual N-glycan structures attached to the specified peptide are labeled. N-glycosylation sites are shown in bold and underlined. Sodium adducts are indicated with an asterisk. MU, Man₂GlcNAc₂; MM, Man₃GlcNAc₂; MMF, Man₃FucGlcNAc₂; Man4 to Man9, Man₄GlcNAc₂ to Man₉GlcNAc₂. N108, Asn-108; N133, Asn-133; N216, Asn-216; N324, Asn-324; N345, Asn-345; N408, Asn-408; N425, Asn-425; N567, Asn-567.