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. 2013 Jun 19;288(31):22270–22280. doi: 10.1074/jbc.M113.475558

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FIGURE 8. — KOR1 N-glycosylation mutants contain complex N-glycans when expressed in plants. A, LC-ESI MS analyses of the glycopeptide containing glycosylation site Asn-425. B, LC-ESI MS analyses of the glycopeptide containing glycosylation site Asn-408. All KOR1-Fc-GFP N-glycosylation mutant variants were transiently expressed in N. benthamiana wild-type plants, purified, and subjected to MS analysis. N-glycosylation sites are shown in bold and underlined. GnGnXF, GlcNAc₂XylFucMan₃GlcNAc₂; GnMXF, GlcNAcXylFucMan₃GlcNAc₂; Man5, Man₅GlcNAc₂; Man6, Man₆GlcNAc₂; Man7, Man₇GlcNAc₂; Man8, Man₈GlcNAc₂; Man9, Man₉GlcNAc₂; N408, Asn-408; N425, Asn-425.