FIGURE 2.

Surface representations of AlgX. A, surface representation of AlgX depicting the N-terminal α/β/α domain in blue and the C-terminal CBM domain in red. The white line indicates the proposed path of alginate across AlgX, between the two domains. When compared with the representations in B and C, it can be seen that the path crosses conserved, mainly electropositive residues. B, electrostatic surface representation of AlgX. The electrostatic potential, calculated using APBS (91–93), is contoured from −7 to 7 kT/e and colored on a gradient from negative (red) to positive (blue). C, representation of the surface residue conservation in eight pseudomonads and A. vinelandii as calculated using ConSurf and colored on a gradient from high (dark pink) to low (cyan) sequence conservation (54, 55).