TABLE 2.

SGNH hydrolase conserved blocks

The conserved active site SGNH hydrolase residues in E. faecalis acylhydrolase/lipase are found in the canonical blocks. The blocks in AlgX are not conserved, and the active site residues are found in a different order in the protein. Residues in boldface type are 100% conserved in SGNH hydrolases. Underlined residues are those found in the canonical block motifs or the equivalent in AlgX.

Classic SGNH hydrolase block	Classic SGNH hydrolase motifs	E. faecalis motifs	AlgX motifs	AlgX signature residues
I	GDSL	GDSI	GTSN	Ser-269
II	NXXSXXGXT	NAGMPGDT	NAVSGGGF	Gly-296
III	GXND	GAND	THYD	Tyr-328
V	DXXHP	DGLHF	DHHW	Asp-174, His-176