FIGURE 8.

The HP1a-CSD-CTE-L165K peptide in conjunction with a pentamer swap between HP2 and PIWI imparts equivalent binding of HP2 and PIWI. A, pentamer exchange between HP2 and PIWI. B and C, fluorescence polarization binding curves for HP1a-CSD-CTE WT and the L165K mutated peptide with HP2 (B) and PIWI (C) using either the WT or pentamer-swapped counterpart in the latter case. D, table of the dissociation constant values demonstrating the equivalent binding of the pentamer-swap peptides to the L165K mutant CDS.