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. 2013 Jun 20;288(31):22378–22386. doi: 10.1074/jbc.M112.444182

FIGURE 2.

FIGURE 2.

IQGAP1 mediates PEITC-induced expression of phase II/antioxidant genes. A, HEK-293 cells were transfected with pcDNA3.1 and IQGAP1 shRNA (shIQGAP1) for 24 h. The down-regulation of IQGAP1 protein was measured. Data are representative of four independent experiments. B, HEK-293 cells transfected with IQGAP1 shRNA (shIQ) or pcDNA3.1 were treated with PEITC for 6 h. mRNA expression of NQO1, UGT1A1, and HO-1 was determined by quantitative PCR. Data represent the mean ± S.D. (n = 3). *, p < 0.05. CON, control. C, HEK-293 cells transfected with IQGAP1 shRNA or pcDNA3.1 were treated with PEITC for 6 h. Protein expression of HO-1 was determined by Western blotting. PEITC-induced HO-1 protein expression in IQGAP1-knockdown cells is expressed relative to wild-type cells and represents the mean ± S.D. (n = 3). *, p < 0.05.