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. 2013 Jun 20;288(31):22378–22386. doi: 10.1074/jbc.M112.444182

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Nrf2 binds to the IQ domain of IQGAP1. A, schematic diagram of the IQGAP1 constructs. CHD, calponin homology domain; GRD, GTPase-activating protein-related domain. B, full-length IQGAP1 proteins, truncated IQGAP1 proteins, and deletion mutants were generated according to the protocol of the TnT® quick coupled transcription/translation system. Dynabeads Protein G (DynaG-beads) and anti-c-Myc antibody were used to pull down proteins that formed complexes with Myc-tagged IQGAP1 proteins. Samples were resolved by SDS-PAGE and probed with anti-Nrf2 antibody. IP, immunoprecipitate; IB, immunoblot. C, Ni-NTA beads were used to pull down proteins that formed complexes with His-tagged Nrf2. Samples were resolved by SDS-PAGE and probed with anti-c-Myc antibody.