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. 2013 Jun 20;288(31):22469–22480. doi: 10.1074/jbc.M113.453043

FIGURE 3.

FIGURE 3.

TGF-β decreases the expression of FOG2 in MMC. A, FOG2 mRNA levels in MMC treated with TGF-β. Significant decrease was detected at 6 and 24 h after TGF-β treatment. Results were normalized with internal control 18 S. Mean ± S.E. (n = 4). B–D, immunoblotting analysis of MMC after treatment with TGF-β (10 ng/ml for 1 h to 24 h). C, TGF-β treatment significantly decreased protein levels of FOG2 at 1–24 h (normalized to β-actin). Mean ± S.E. (n = 4). D, phosphorylation of Akt (p-Akt) (normalized to total Akt) significantly increased with TGF-β treatment. Mean ± S.E. (n = 4). ** and * indicate p < 0.01 and p < 0.05, respectively versus S.D.