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. 2013 Jun 20;288(31):22469–22480. doi: 10.1074/jbc.M113.453043

FIGURE 6.

FIGURE 6.

FOG2 is down-regulated by mimics of miR-200b (200b-M) and miR-200c (200c-M) in MMC. A, levels of miR-200b/c in MMC transfected with miR-200b and miR-200c mimics. Transfection with 200b-M or 200c-M significantly increased miR-200b and miR-200c levels, respectively, relative to NC-M oligonucleotides. Results were normalized with internal control U6. Mean ± S.E. (n = 3). B, expression of FOG2 mRNA in MMC transfected with miR-200b and miR-200c mimics. Transfection with 200b-M or 200c-M significantly decreased FOG2 mRNA levels. Results were normalized with internal control 18 S. Mean ± S.E. (n = 4). C–E, immunoblotting analysis of MMC after transfection with 200b-M and 200c-M. D, FOG2 protein levels normalized to β-actin were significantly decreased after transfection with 200b-M or 200c-M. Mean ± S.E. (n = 4). E, transfection with 200b-M or 200c-M significantly increased phosphorylation of Akt (p-Akt) normalized to total Akt. Mean ± S.E. (n = 4). ** and * indicate p < 0.01 and p < 0.05, respectively.