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. 2013 Jun 20;288(31):22469–22480. doi: 10.1074/jbc.M113.453043

FIGURE 8.

FIGURE 8.

FOG2 down-regulation phosphorylates Akt as well as ERK in a PI3K-dependent manner in MMC. A–D, immunoblotting analysis of MMC after siFOG2 transfection followed by LY-294002 (LY) and MK-2206 (MK) treatment. siFOG2-transfected MMC were treated with either 20 μm LY or 1 μm MK for 24 h. B, FOG2 protein levels significantly decreased after siFOG2 transfection (normalized to β-actin). Mean ± S.E. (n = 4). C, transfection with siFOG2 increased phosphorylation of Akt. This increase in Akt phosphorylation was significantly attenuated by LY and MK treatment (normalized to total Akt). Mean ± S.E. (n = 4). D, siFOG2 transfection significantly increased phosphorylation of ERK. This increased ERK phosphorylation was significantly decreased by LY, but not by MK treatment (normalized to total ERK). Mean ± S.E. (n = 4). ** and * indicate p < 0.01 and p < 0.05, respectively.