FIGURE 3.

Tubastatin A and TSA stabilized microtubules against nocodazole-induced disassembly. A, MCF-7 cells were treated with either vehicle, 15 μm tubastatin A (TBA), 240 nm TSA, or 200 nm nocodazole (Nz) individually or with a combination of nocodazole (200 nm) and tubastatin A (15 μm) or nocodazole (200 nm) and TSA (240 nm) for 24 h and processed for immunostaining with antibodies against α-tubulin (green) and acetylated tubulin (red). Scale bar, 10 μm. B, MCF-7 cells were treated with either vehicle, 15 μm tubastatin A (TBA), 240 nm TSA, 200 nm nocodazole (Nz) individually or with a combination of nocodazole (200 nm) and tubastatin A (15 μm) or nocodazole (200 nm) and TSA (240 nm) for 24 h and immunostained with antibodies against α-tubulin (red) and detyrosinated tubulin (green). Scale bar, 10 μm.