FIGURE 2.

C1qa deficiency prevents altered glucose homeostasis after 8 weeks of high fat diet. C57BL/6J and C1qa^−/− mice were allowed free access to a high fat diet (HFD) or low fat diet (LFD) for 8 weeks. Mice were fasted for 6 h prior to blood collection and euthanasia. A, fasting blood glucose was measured from a tail nick using a standard glucometer. B, fasting insulin was measured in plasma samples using a commercially available ELISA kit. C, HOMA-IR was calculated using the formula, (fasting glucose (mg/dl) × fasting insulin (ng/ml))/405. D, for glucose tolerance tests, mice received 20% d-glucose solution (2 mg/kg of bodyweight) by intraperitoneal injection. Blood glucose was measured at base line and at 15, 30, 60, and 120 min. For all graphs, values represent means ± S.E. (error bars). A–C, results labeled with different lowercase letters are significantly different from one another (p < 0.05), n = 4–6. D, *, p < 0.05 compared with C1qa^−/− mice.