FIGURE 4.

miR-200s modulate the activation of AKT/GSK pathway and the glycogenesis in the hepatocytes. The levels of miR-200s were detected in the NCTC 1469 cells (negative control (NC)) transfected with miR-200 mimics (A) and miR-200 inhibitors (B). The effects of miR-200s on the phosphorylation of AKT and GSK3 in both insulin-treated and -untreated NCTC1469 cells were analyzed (C). The activation of the AKT/GSK pathway and the content of glycogen were measured in the NCTC 1469 cells transfected with miR-200 mimics (D) and miR-200 inhibitors (E). Data represent the means ± S.E. (error bars), n = 3 independent experiments. *, p < 0.05 versus control; **, p < 0.01 versus control. p-AKT and p-GSK, phospho-AKT and -GSK, respectively.