FIGURE 7.
Binding of hnRNP L RRM34 to its in vivo target RNA. A, electrophoretic mobility gel shift data for binding of 34-nt RNA to hnRNP L RRM34. The RNA sequence is shown above. Activation-responsive sequence motifs (underlined and in bold) of CD45 exon 5, which are separated by a 21-nt spacer, represent binding sites for individual hnRNP L RRM domains. B, ITC measuring binding of hnRNP L RRM34 to 34-nt RNA (top, raw titration data; bottom, integrated heat measurements). The curve was fitted using a single-site binding model with KD and n indicated.