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Effect of iron on AtFer1 response to phosphate starvation. Plants were grown on complete medium for 10 days and then transferred on Pi-deficient medium (−Pi), or kept in complete medium (+Pi) for 7 days. Iron starvation was applied 2 days before harvesting. Relative transcript levels were assayed by RT-qPCR relative to an internal control (At1g13320) using the 2−ΔΔCP method. Values presented are the means of 3 points ± S.D. A, expression in leaves. B, expression in roots.
