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. 2013 Jun 20;288(31):22670–22680. doi: 10.1074/jbc.M113.482281

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Metal content and iron-related genes expression in phr1phl1. Plants were grown on complete medium for 10 days and then transferred on P_i-deficient medium (−P_i), or kept in complete medium (+P_i) for 7 days. A, leaves were dried, digested with HNO₃, and diluted with ultrapure water to 1% HNO₃. Metal content was then measured by ICPMS. Values are means of 3 points ± S.D., nd: not detectable. B, plants were grown on complete medium for 10 days and then transferred on P_i-deficient medium (black bars), or kept in complete medium (gray bars) for 7 days. RNA was prepared from leaves. Relative transcript levels were assayed by RT-qPCR relative to an internal control (At1g13320) using the 2^−ΔΔCP method. Values are presented as the mean of 3 independent biological repeats ± S.D.