TABLE 1.
Stable binary contacts between amino acid residues in hole ‘a’ and knob ‘A’ and between residues in hole ‘b’ and knob ‘B’ that stabilize the non-covalent A:a and B:b knob-hole bonds in fibrin
| Model systems | Residues involved in A:a and B:b knob-hole interactions |
|
|---|---|---|
| Holes ‘a’ and ‘b’ | Knobs ‘A’ and ‘B’ | |
| Aa1 and Aa2 (pH 7) | γPhe295, γAsp330, γCys339, γHis340, γArg375 | Gly1 in GPRP |
| γPhe295, γAsp297, γAsp298, γThr305, γAsp364, γArg375 | Pro2 in GPRP | |
| γPhe322, γCys326, γGln329, γAsp330, γCys339, γTyr363 | Arg3 in GPRP | |
| Aa3 and Aa4 (pH 5) | γPhe295, γAsp297, γThr305, γAsp364, γArg375 | Gly1 in GPRP |
| γPhe295, γAsp297, γAsp298, γAsp301, γThr305 | Pro2 in GPRP | |
| γPhe322, γCys326, γGln329, γAsp330, γCys339, γTyr363, γAsp364 | Arg3 in GPRP | |
| Bb1 and Bb2 (pH 7) | βAsp398, βCys407, βHis408, βAsp432, βMet438, βSer443 | Gly1 in GHRP |
| βLeu360, βAsn364, βThr308, βMet438, βSer443 | His2 in GHRP | |
| βCys394, βGlu397, βAsp398, βCys407, βThr431 | Arg3 in GHRP | |
| Bb3 and Bb4 (pH 5) | βAsp398, βCys407, βHis408, βAsp432, βMet438, βSer443 | Gly1 in GHRP |
| βLeu360, βAsn364 | His2 in GHRP | |
| βCys394, βGlu397, βAsp398, βCys407 | Arg3 in GHRP | |