Electron microscopic images of BAEC treated without or with EGCG.
A and B, BAEC were treated without (upper panels) or with (lower panels) 10 μm EGCG for 2 h. Autophagosomes (thin solid arrows), autophagolysosomes (white triangle), and phagophore (black arrow) are indicated. C and D, areas of autophagosome and autophagolysosome were calculated as described under “Experimental Procedures.” Data are mean ± S.E. (***, p < 0.001, versus nontreated (NT) cells).