FIGURE 7.
Palmitate-induced inhibition of autophagic flux, which was opposed by co-treatment with EGCG. BAEC were treated with BSA (0.1%) or palmitate (200 μm)/BSA conjugate in the presence or absence of EGCG (10 μm) for 4 h. Lysosomal inhibitor (20 mm NH4Cl, 200 μm leupeptin) was treated 1 h prior to cell harvest. Cell lysate was analyzed by immunoblotting for LC3-II formation. Three independent experiments were performed, and density of LC3-II/LC3-I was quantified. Data are mean ± S.E. (*, p < 0.05; ***, p < 0.001). ns, not significant.